COX5A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32550

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse

Predicted:

Bovine (93%), Canine (94%), Chimpanzee (99%), Rat (93%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COX5A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (86%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX5A Antibody - BSA Free

Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: COX5A Antibody [NBP1-32550]

Immunocytochemistry/ Immunofluorescence: COX5A Antibody [NBP1-32550]

Immunocytochemistry/Immunofluorescence: COX5A Antibody [NBP1-32550] - A431.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Analysis of paraffin-embedded zebrafish tissue, using COX5A antibody [N1C3] at 1:300 dilution.
Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550]

Western Blot: COX5A Antibody [NBP1-32550] - Sample (30 ug of whole cell lysate) A: zebrafish eye15% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Cal27 xenograft, using COX5A antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Mouse prostate. COX5A antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550]

Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Mouse cervix. COX5A antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for COX5A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:10

Western Blot

1:1000-1:10000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, antibody dilution of 1:10

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COX5A

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq]

Alternate Names

COX, COX-VA, Cytochrome c oxidase polypeptide Va, cytochrome c oxidase polypeptide, mitochondrial, cytochrome c oxidase subunit 5A, mitochondrial, cytochrome c oxidase subunit Va, mitochondrial cytochrome c oxidase subunit Va, VA

Entrez Gene IDs

9377 (Human)

Gene Symbol

COX5A

UniProt

P20674 (Human)

Additional COX5A Products

Product Documents for COX5A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COX5A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for COX5A Antibody - BSA Free

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Protocols

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