CPSF2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15989

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Zebrafish

Predicted:

Bovine (100%), Chicken (100%), Monkey (100%), Mouse (100%), Rat (100%), Xenopus (98%), Zebrafish (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CPSF2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPSF2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%), Rhesus Monkey (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CPSF2 Antibody - BSA Free

Western Blot: CPSF2 Antibody [NBP2-15989]

Western Blot: CPSF2 Antibody [NBP2-15989]

Western Blot: CPSF2 Antibody [NBP2-15989] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
CPSF2 Antibody

Immunohistochemistry: CPSF2 Antibody [NBP2-15989] -

Immunohistochemistry: CPSF2 Antibody [NBP2-15989] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using CPSF2 antibody [N1N3] (NBP2-15989) at 1:200 dilution.
CPSF2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CPSF2 Antibody [NBP2-15989] -

CPSF2 antibody [N1N3] detects CPSF2 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CPSF2 protein stained by CPSF2 antibody [N1N3] (NBP2-15989) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for CPSF2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CPSF2

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.

Alternate Names

cleavage and polyadenylation specific factor 2, 100kDa, Cleavage and polyadenylation specificity factor 100 kDa subunit, cleavage and polyadenylation specificity factor subunit 2, CPSF 100 kDa subunit, CPSF 100kDa subunit, CPSF100, KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Gene Symbol

CPSF2

UniProt

Q9P2I0 (Human)

Additional CPSF2 Products

Product Documents for CPSF2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CPSF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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