Cre Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56135

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Cre/lox System

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, IHC-F

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Full-length recombinant bacteriophage protein CRE was used as immunogen, GenBank no. AAQ14086.1.

Reactivity Notes

Not yet tested in other species.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cre Antibody - BSA Free

Immunohistochemistry-Frozen: Cre Antibody [NB100-56135]

Immunohistochemistry-Frozen: Cre Antibody [NB100-56135]

Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-Cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum. Image from verified customer review.
Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135]

Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135]

Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135] - Analysis of tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. Arrows point to negative glia.

Applications for Cre Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56135 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cre

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. This antibody recognizes the recombinase protein, Cre. Cre is a 343 amino acid protein, GenBank no. AAQ14086.1.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cre Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cre Antibody - BSA Free

Customer Reviews for Cre Antibody - BSA Free (2)

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Customer Images

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  • Cre Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse inner ear
    Species: Mouse
    Verified Customer | Posted 08/27/2018
    cochlea cross-section of Cre+/-mouse, stained for cre (green) and counterstained for F-actin (red).
    Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum
    Cre Antibody - BSA Free NB100-56135
  • Cre Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse brain, Mouse embryo and Transgenic Mouse Brain (40um)
    Species: Mouse
    Verified Customer | Posted 04/02/2017

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Protocols

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FAQs for Cre Antibody - BSA Free

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  • Q: A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?

    A:

    With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

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