Creatine kinase MT 1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31543

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Zebrafish

Predicted:

Bovine (98%), Rabbit (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Creatine kinase MT 1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT 1B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (88%).

Localization

Mitochondrion inner membrane, Peripheral membrane protein, Intermembrane side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Creatine kinase MT 1B Antibody - BSA Free

Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543]

Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543]

Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543] - Sample(30 ug whole cell lysate) A:H1299 B:HeLa S310% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: Creatine kinase MT 1B Antibody [NBP1-31543]

Immunohistochemistry-Paraffin: Creatine kinase MT 1B Antibody [NBP1-31543]

Immunohistochemistry-Paraffin: Creatine kinase MT 1B Antibody [NBP1-31543] - Paraffin-embedded A549 xenograft, using antibody at 1:300 dilution.
Creatine kinase MT 1B Antibody

Immunohistochemistry: Creatine kinase MT 1B Antibody [NBP1-31543] -

Immunohistochemistry: Creatine kinase MT 1B Antibody [NBP1-31543] - Creatine kinase MT 1B antibody detects Creatine kinase MT 1B protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Creatine kinase MT 1B antibody (NBP1-31543) dilution: 1:100.

Applications for Creatine kinase MT 1B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Creatine kinase MT 1B

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1B, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1B, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, UMTCK, U-MtCK

Gene Symbol

CKMT1B

Additional Creatine kinase MT 1B Products

Product Documents for Creatine kinase MT 1B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Creatine kinase MT 1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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