CRISPR-Cpf1 Antibody (2D5-6G11) - BSA Free

Novus Biologicals | Catalog # NBP2-78819

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria, Bacterial

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D5-6G11

Format

BSA Free
View all Cpf1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an N-terminal portion of the Lachnospiraceae bacterium CRISPR-Cpf1 protein

Specificity

This antibody is specific for Lachnospiraceae bacterium CRISPR-Cpf1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CRISPR-Cpf1 Antibody (2D5-6G11) - BSA Free

Western Blot: CRISPR-Cpf1 Antibody (2D5-6G11)BSA Free [NBP2-78819]

Western Blot: CRISPR-Cpf1 Antibody (2D5-6G11)BSA Free [NBP2-78819]

Western Blot: CRISPR-Cpf1 Antibody (2D5-6G11) [NBP2-78819] - Lachnospiracea Cpf1 protein and negative control protein from human HeLa and mouse Neuro2A was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Lachnospiraceae Cpf1 in block and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Applications for CRISPR-Cpf1 Antibody (2D5-6G11) - BSA Free

Application
Recommended Usage

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cpf1

Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) is an adaptive immune system in bacteria which provides protection against mobile genetic elements from viruses such as, transposable elements and conjugative plasmids. CRISPR clusters are known to have sequences which are complementary to antecedent mobile elements and target invading nucleic acids, and these sequences are transcribed/processed into CRISPR RNA. CRISPR possess endonuclease activity on pre-crRNA and dsDNA, using different active sites, and in a complexed form, crRNA displaces the non-target DNA strand and guides endo-nucleolytic cleavage of both strands of the DNA. CRISPR associated Cas12a (CRISPR-Cas12a), also known as Cpf1, is emerging as a genome editing tool with different target specificity, when compared to CRISPR-Cas9. CRISPR-Cas12a-assisted recombineering can be used for genetic manipulation of plasmids, plasmid curing and for a more precise selection of target sites for the introduction of desired mutations (point mutations, deletions, insertions, and replacements). In cells subjected to genetic manipulation using this tool, CRISPR-Cas12a antibodies are needed to verify transfection efficiency, CRISPR-Cas12a expression, CRISPR-Cas12a target binding and subcellular localization. Novus Biologicals' CRISPR-Cpf1 antibody (NBP2-62735) is the first-ever commercially launched antibody to meet this need. This antibody is generated against a partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1], and validated for Western blot using a partial recombinant CRISPR-Cpf1 protein (12kDa), making it a great tool for a direct detection of CRISPR-Cpf1/CRISPR-Cas12a complex (at protein level) superior to the use of epitope-tag based validation approaches.

Long Name

CRISPR-associated Endonuclease Cas12a

Alternate Names

Cas12a, anti-cas12a, anti-cas12a antibody, anti-CPF1, anti-CRISPR-Cas12a, anti-CRISPR-Cpf1

Additional Cpf1 Products

Product Documents for CRISPR-Cpf1 Antibody (2D5-6G11) - BSA Free

Certificate of Analysis

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Product Specific Notices for CRISPR-Cpf1 Antibody (2D5-6G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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