Key Product Details

Species Reactivity

Bacterial

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cpf1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CRISPR-Cpf1 Antibody - BSA Free (NBP2-62735) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CRISPR-Cpf1 Antibody - BSA Free

Western Blot: CRISPR-Cpf1 AntibodyBSA Free [NBP2-62735]

Western Blot: CRISPR-Cpf1 AntibodyBSA Free [NBP2-62735]

Western Blot: CRISPR-Cpf1 Antibody [NBP2-62735] - Partial recombinant Cpf1 protein (Ca. 12 kDa) was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Cpf1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Lane 1, 10 ng, Lane 2. 5 ng.

Applications for CRISPR-Cpf1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cpf1

Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) is an adaptive immune system in bacteria which provides protection against mobile genetic elements from viruses such as, transposable elements and conjugative plasmids. CRISPR clusters are known to have sequences which are complementary to antecedent mobile elements and target invading nucleic acids, and these sequences are transcribed/processed into CRISPR RNA. CRISPR possess endonuclease activity on pre-crRNA and dsDNA, using different active sites, and in a complexed form, crRNA displaces the non-target DNA strand and guides endo-nucleolytic cleavage of both strands of the DNA. CRISPR associated Cas12a (CRISPR-Cas12a), also known as Cpf1, is emerging as a genome editing tool with different target specificity, when compared to CRISPR-Cas9. CRISPR-Cas12a-assisted recombineering can be used for genetic manipulation of plasmids, plasmid curing and for a more precise selection of target sites for the introduction of desired mutations (point mutations, deletions, insertions, and replacements). In cells subjected to genetic manipulation using this tool, CRISPR-Cas12a antibodies are needed to verify transfection efficiency, CRISPR-Cas12a expression, CRISPR-Cas12a target binding and subcellular localization. Novus Biologicals' CRISPR-Cpf1 antibody (NBP2-62735) is the first-ever commercially launched antibody to meet this need. This antibody is generated against a partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1], and validated for Western blot using a partial recombinant CRISPR-Cpf1 protein (12kDa), making it a great tool for a direct detection of CRISPR-Cpf1/CRISPR-Cas12a complex (at protein level) superior to the use of epitope-tag based validation approaches.

Long Name

CRISPR-associated Endonuclease Cas12a

Alternate Names

Cas12a, anti-cas12a, anti-cas12a antibody, anti-CPF1, anti-CRISPR-Cas12a, anti-CRISPR-Cpf1

UniProt

A0Q7Q2 (Bacteria)

Additional Cpf1 Products

Product Documents for CRISPR-Cpf1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CRISPR-Cpf1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for CRISPR-Cpf1 Antibody - BSA Free (NBP2-62735):

CRISPR-Cpf1 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10 ng of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute anti-CRISPR-Cpf1 primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.


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