CrkL Antibody (5E12)

Name: CrkL Antibody (5E12)
Brand: Novus Biologicals
SKU: NBP2-37586
Price: 139 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37586

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 5E12

View all CrkL Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CrkL expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CrkL Antibody (5E12)

Western Blot: CrkL Antibody (5E12)BSA Free [NBP2-37586]

Western Blot: CrkL Antibody (5E12) [NBP2-37586] - Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recombinant protein. (Expected MW is 60 kDa)

Flow Cytometry: CrkL Antibody (5E12) - BSA Free [NBP2-37586]

Flow Cytometry: CrkL Antibody (5E12) [NBP2-37586] - Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and negative control (red).

ELISA: CrkL Antibody (5E12) - BSA Free [NBP2-37586]

ELISA: CrkL Antibody (5E12) [NBP2-37586] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CrkL Antibody (5E12)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CrkL

CrkL is a widely-expressed 39 kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoetic origin. Through its SH2 and SH3 domains CrkL initiates interactions with numerous downstream proteins such as CBL, Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. It is involved in processes such as adhesion, migration and immune responses. Furthermore, CrkL plays an essential role in Chronic Myelogenous Leukemia and is constitutively phosphorylated by the Bcr-Abl kinase in this disease.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Gene Symbol

CRKL

Additional CrkL Products

Product Documents for CrkL Antibody (5E12)

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Certificate of Analysis

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Product Specific Notices for CrkL Antibody (5E12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

Interferons

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