CRYZ Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35279

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRYZ Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CRYZ (NP_001123514.1).

Sequence:
MATGQKLMRAVRVFEFGGPEVLKLRSDIAVPIPKDHQVLIKVHACGVNPVETYIRSGTYSRKPLLPYTPGSDVAGVIEAVGDNASAFKKGDRVFTSSTISGGYAEYALAADHTVYKLPEKLDFKQGAAIGIPYFTAYRALIHSACVKAGESVLVHGASGGVGLAACQIARAYGLKILGTAGTEEGQKIVLQNGAHEVFNHREVNYIDKIKKYVGEKGIDIIIEMLANVNLSKDLSLLSHGGRVIVVGSRGTIEINPRDTMAKESSIIGVTLFSSTKEEFQQYAAALQAGMEIGWLKPVIGSQYPLEKVAEAHENIIHGSGATGKMILLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRYZ Antibody - BSA Free

CRYZ Antibody

Western Blot: CRYZ Antibody [NBP3-35279] -

Western Blot: CRYZ Antibody [NBP3-35279] - Western blot analysis of various lysates using CRYZ Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
CRYZ Antibody

Immunocytochemistry/ Immunofluorescence: CRYZ Antibody [NBP3-35279] -

Immunocytochemistry/ Immunofluorescence: CRYZ Antibody [NBP3-35279] - Immunofluorescence analysis of U-2 OS cells using CRYZ Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
CRYZ Antibody

Immunocytochemistry/ Immunofluorescence: CRYZ Antibody [NBP3-35279] -

Immunocytochemistry/ Immunofluorescence: CRYZ Antibody [NBP3-35279] - Immunofluorescence analysis of L929 cells using CRYZ Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for CRYZ Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CRYZ

Alternate Names

crystallin, zeta (quinone reductase), DKFZp779E0834, EC 1.6.5.5, FLJ41475, NADPH:quinone reductase, quinone oxidoreductase, Zeta-crystallin

Gene Symbol

CRYZ

Additional CRYZ Products

Product Documents for CRYZ Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CRYZ Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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