CSK Antibody

Novus Biologicals | Catalog # NB100-808

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CSK Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EQLEHIKTHELHL corresponding to C-Terminus according to NP_004374.1, NP_001120662.1.

Localization

Nuclear

Specificity

Reported variants represent identical protein: NP_004374.1; NP_001120662.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CSK Antibody

Western Blot: CSK Antibody [NB100-808]

Western Blot: CSK Antibody [NB100-808]

Western Blot: CSK Antibody [NB100-808] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Applications for CSK Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Approx 48kDa band observed in lysates of Jurkat, K562 and MOLT4 (calculated MW of 50.7 kDa according to NP_004374.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CSK

C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Entrez Gene IDs

1445 (Human); 12988 (Mouse)

Gene Symbol

CSK

UniProt

NP_004374.1 (Human)

Additional CSK Products

Product Documents for CSK Antibody

Certificate of Analysis

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Product Specific Notices for CSK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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