Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2B6
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Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human CTAG1B expression vector
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CTAG1B Antibody (OTI2B6)
![Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Western-Blot-NBP1-47714-img0017.jpg "Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).![Immunocytochemistry/ Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Immunocytochemistry-Immunofluorescence-NBP1-47714-img0013.jpg "Immunocytochemistry/ Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunocytochemistry/ Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of 293T cells transfected by pCMV6-ENTRY CTAG1B(RC213318) using anti-CTAG1B antibody (green, upper left; DAPI/blue, lower left; merged, upper right). 293T cells transfected with empty vector served as a negative control (merged, lower right). (1:100)![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0018.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP1-47714, Dilution 1:50)![Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Flow-Cytometry-NBP1-47714-img0016.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living A375 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)![Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Western-Blot-NBP1-47714-img0001.jpg "Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0002.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0003.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0004.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0005.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0006.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0007.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0008.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CTAG1B mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-2B6-Immunohistochemistry-Paraffin-NBP1-47714-img0009.jpg "Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody.![Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Flow-Cytometry-NBP1-47714-img0014.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)![Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]](https://resources.rndsystems.com/images/products/CTAG1B-Antibody-OTI2B6-Flow-Cytometry-NBP1-47714-img0015.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]")
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714]
Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living NCI-H1299 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)Applications for CTAG1B Antibody (OTI2B6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:5000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CTAG1B
Long Name
Cancer/Testis Antigen 1B
Alternate Names
CT6.1, CTAG1, LAGE-2, NY-ESO-1
Entrez Gene IDs
1485 (Human)
Gene Symbol
CTAG1B
UniProt
Additional CTAG1B Products
Product Documents for CTAG1B Antibody (OTI2B6)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CTAG1B Antibody (OTI2B6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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