CTDSPL Antibody

Novus Biologicals | Catalog # NBP1-20903

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CTDSPL Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QCNVSLKKQRSRS corresponding to internal region according to NP_001008393.1, NP_005799.2.

Epitope

internal region

Specificity

This antibody is expected to recognise both isoforms (NP_001008393.1; NP_005799.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Scientific Data Images for CTDSPL Antibody

CTDSPL Antibody

Immunocytochemistry/Immunofluorescence: CTDSPL Antibody [NBP1-20903] -

Immunocytochemistry/Immunofluorescence: CTDSPL Antibody [NBP1-20903] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CTDSPL Antibody

Flow Cytometry: CTDSPL Antibody [NBP1-20903] -

Flow Cytometry: CTDSPL Antibody [NBP1-20903] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for CTDSPL Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary testing showed a band at approx 28kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2ug/ml antibody staining (calculated Mwt of 29.9kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CTDSPL

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells

Alternate Names

C3orf8, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase-like, CTD small phosphatase-like protein, CTDSP-like, EC 3.1.3, hYA22, NIF1, NIFL, NIF-like protein, NLI-interacting factor 1, Nuclear LIM interactor-interacting factor 1, Protein YA22, PSR1, RB protein serine phosphatase from chromosome 3, RBSP3EC 3.1.3.16, SCP3chromosome 3 open reading frame 8, Small CTD phosphatase 3, Small C-terminal domain phosphatase 3, YA22

Entrez Gene IDs

10217 (Human)

Gene Symbol

CTDSPL

UniProt

NP_001008393.1 (Human)

Additional CTDSPL Products

Product Documents for CTDSPL Antibody

Certificate of Analysis

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Product Specific Notices for CTDSPL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CTDSPL Antibody

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Protocols

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