CTGF/CCN2 Antibody (CL5339) - BSA Free
Novus Biologicals | Catalog # NBP2-61415
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL5339
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P29279, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse CTGF/CCN2 Antibody (CL5339) - BSA Free (NBP2-61415) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CTGF/CCN2 Antibody (CL5339) - BSA Free
![Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Western-Blot-NBP2-61415-img0007.jpg "Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0013.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0008.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0009.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0010.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0011.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]](https://beta-resources.rndsystems.com/images/products/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0012.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]")
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.Applications for CTGF/CCN2 Antibody (CL5339) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CTGF/CCN2
CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.
Long Name
Connective Tissue Growth Factor
Alternate Names
CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2
Gene Symbol
CCN2
Additional CTGF/CCN2 Products
Product Documents for CTGF/CCN2 Antibody (CL5339) - BSA Free
Product Specific Notices for CTGF/CCN2 Antibody (CL5339) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CTGF/CCN2 Antibody (CL5339) - BSA Free
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Q: We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
A: Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
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