CTGF/CCN2 Antibody

Novus Biologicals | Catalog # NBP2-16025

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Baboon, Bovine

Cited:

Human, Mouse

Predicted:

Chicken (94%), Porcine (95%), Primate (99%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CTGF/CCN2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTGF/CCN2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%).

Localization

Secreted; extracellular space; extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CTGF/CCN2 Antibody

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CTGF/CCN2 Antibody [NBP2-16025]

Western Blot: CTGF/CCN2 Antibody [NBP2-16025]

Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CTGF/CCN2 Antibody

Western Blot: CTGF/CCN2 Antibody [NBP2-16025] -

HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (NBP2-16025) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CTGF/CCN2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-16025 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CTGF/CCN2

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Gene Symbol

CCN2

UniProt

P29279

Additional CTGF/CCN2 Products

Product Documents for CTGF/CCN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CTGF/CCN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CTGF/CCN2 Antibody

Customer Reviews for CTGF/CCN2 Antibody (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • CTGF/CCN2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 3 human pancreas cancer cell lines
    Species: Human
    Verified Customer | Posted 01/08/2019
    We used this antibody on PANC-1 cells lysates. There are two bands between 35 and 50 kd and several strong non-specific bands bigger than 50 kd.
    CTGF/CCN2 Antibody NBP2-16025

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CTGF/CCN2 Antibody

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  • Q: We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.

    A: Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

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