Cultrex Collagen IV Cell Invasion Assay, 96-well Summary
Why Use Cultrex Collagen IV Cell Invasion Assay, 96-well?
The Cultrex Collagen IV Cell Invasion Assays provide systems where the researcher can determine the optimal Collagen IV coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through Collagen IV may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex Collagen IV Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.
Cultrex Mouse Collagen IV is purified from Engelbreth-Holm-Swarm (EHS) tumor. Collagen IV is a component of the basement membrane which is a continuous sheet of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.
• 10X Coating Buffer
• 5X Collagen IV Solution
• Calcein AM
• Cell Invasion Chamber
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnositic use.
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells. The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.
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