Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well
Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well SummaryProvides a tool for assessing cell invasion through a complex extracellular matrix.
• Standardized assay for extracellular matrix invasion
• Available in 24 and 96 well formats
• Allows optimization for specific cell lines
Why Use Cultrex BME Cell Invasion Assay, 96-well?
The Cultrex BME Cell Invasion Assays provide systems where the researcher can determine the optimal BME coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through BME may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.
Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
• 5X Cultrex Basement Membrane Extract (BME) Coating Solution
Tested to support EMT-induced cell invasion and migration using A549 human lung carcinoma cell lines.
For research use only. Not for diagnostic use.
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells. The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.
Citations for Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well
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Inhibition of triple negative breast cancer-associated inflammation and progression by N- acylethanolamine acid amide hydrolase (NAAA)
Authors: O Benchama, MS Malamas, K Praveen, EC Ethier, MK Williams, A Makriyanni, HK Avraham
Scientific Reports, 2022-12-23;12(1):22255. 2022-12-23
Doxycycline inhibits the progression of metastases in early-stage osteosarcoma by downregulating the expression of MMPs, VEGF and ezrin at primary sites
Authors: AC Hadjimicha, AF Foukas, E Papadimitr, A Kaspiris, C Peristiani, I Chaniotaki, M Kotsari, A Pergaris, S Theocharis, P Sarantis, M Christopou, A Psyrri, AF Mavrogenis, OD Savvidou, PJ Papagelopo, A Armakolas
Cancer treatment and research communications, 2022-08-05;32(0):100617. 2022-08-05
Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1's pro-metastatic functions in esophageal cancer
Authors: G Li, HW Qi, HG Dong, P Bai, M Sun, HY Liu
Cancer Cell Int., 2020-07-30;20(0):355. 2020-07-30
Phosphorylated Rasal2 facilitates breast cancer progression
Authors: X Wang, C Qian, Y Yang, MY Liu, Y Ke, ZM Qian
EBioMedicine, 2019-11-21;0(0):. 2019-11-21
MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
Sci Rep, 2019-07-05;9(1):9776. 2019-07-05
PYK2 promotes HER2-positive breast cancer invasion
Authors: SI Al-Juboori, J Vadakekola, S Idri, S Wagner, D Zafeiris, JR Pearson, R Almshayakh, M Caraglia, V Desiderio, AK Miles, DJ Boocock, GR Ball, T Regad
J. Exp. Clin. Cancer Res., 2019-05-22;38(1):210. 2019-05-22
Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells
Authors: M Paku?a, A Witucka, P Uruski, A Radziemski, R Moszy?ski, D Szpurek, K Maksin, A Wo?niak, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
Sci Rep, 2019-05-20;9(1):7587. 2019-05-20
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
Int J Mol Sci, 2019-01-02;20(1):. 2019-01-02
Antineoplastic effects of selective CDK9 inhibition with atuveciclib on cancer stem-like cells in triple-negative breast cancer
Authors: D Brisard, F Eckerdt, LA Marsh, GT Blyth, S Jain, M Cristofani, D Horiuchi, LC Platanias
Oncotarget, 2018-12-18;9(99):37305-37318. 2018-12-18
Thyroid cancer stem-like cell exosomes: regulation of EMT via transfer of lncRNAs
Authors: H Hardin, H Helein, K Meyer, S Robertson, R Zhang, W Zhong, RV Lloyd
Lab. Invest., 2018-07-02;98(9):1133-1142. 2018-07-02
MicroRNA-182 targets protein phosphatase 1 regulatory inhibitor subunit 1C in glioblastoma
Authors: L Liu, X Zhang, C Nan, Z Zhao, S Ma, W Li, H Hu, Z Liang
Oncotarget, 2017-09-27;8(70):114677-114684. 2017-09-27
Metformin inhibits gastric cancer cells metastatic traits through suppression of epithelial-mesenchymal transition in a glucose-independent manner
Authors: S Valaee, MM Yaghoobi, M Shamsara
PLoS ONE, 2017-03-23;12(3):e0174486. 2017-03-23
Heterogeneous stromal signaling within the tumor microenvironment controls the metastasis of pancreatic cancer
Cancer Res., 2016-11-07;0(0):. 2016-11-07
Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells
Sci Rep, 2016-09-08;6(0):33132. 2016-09-08
Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes.
Authors: Smith G, Howell G, Phillips C, Muench S, Ponnambalam S, Harrison M
J Biol Chem, 2016-02-24;291(16):8500-15. 2016-02-24
The MCT4 Gene: A Novel, Potential Target for Therapy of Advanced Prostate Cancer.
Authors: Choi S, Xue H, Wu R, Fazli L, Lin D, Collins C, Gleave M, Gout P, Wang Y
Clin Cancer Res, 2016-01-11;22(11):2721-33. 2016-01-11
Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels.
Authors: Fujimura K, Choi S, Wyse M, Strnadel J, Wright T, Klemke R
J Biol Chem, 2015-10-19;290(50):29907-19. 2015-10-19
Semaphorin 3D autocrine signaling mediates the metastatic role of annexin A2 in pancreatic cancer.
Authors: Foley K, Rucki A, Xiao Q, Zhou D, Leubner A, Mo G, Kleponis J, Wu A, Sharma R, Jiang Q, Anders R, Iacobuzio-Donahue C, Hajjar K, Maitra A, Jaffee E, Zheng L
Sci Signal, 2015-08-04;8(388):ra77. 2015-08-04
Reciprocal regulation of amino acid import and epigenetic state through Lat1 and EZH2.
Authors: Dann S, Ryskin M, Barsotti A, Golas J, Shi C, Miranda M, Hosselet C, Lemon L, Lucas J, Karnoub M, Wang F, Myers J, Garza S, Follettie M, Geles K, Klippel A, Rollins R, Fantin V
EMBO J, 2015-05-15;34(13):1773-85. 2015-05-15
1alpha,25-Dihydroxyvitamin D3 Inhibits Esophageal Squamous Cell Carcinoma Progression by Reducing IL6 Signaling.
Authors: Chen P, Hsieh C, Wu C, Yen T, Lin P, Chen W, Chen M
Mol Cancer Ther, 2015-03-30;14(6):1365-75. 2015-03-30
The roles of the epithelial-mesenchymal transition marker PRRX1 and miR-146b-5p in papillary thyroid carcinoma progression.
Authors: Hardin H, Guo Z, Shan W, Montemayor-Garcia C, Asioli S, Yu X, Harrison A, Chen H, Lloyd R
Am J Pathol, 2014-06-17;184(8):2342-54. 2014-06-17
Hypermethylation of the GABRE~miR-452~miR-224 promoter in prostate cancer predicts biochemical recurrence after radical prostatectomy.
Authors: Kristensen H, Haldrup C, Strand S, Mundbjerg K, Mortensen M, Thorsen K, Ostenfeld M, Wild P, Arsov C, Goering W, Visakorpi T, Egevad L, Lindberg J, Gronberg H, Hoyer S, Borre M, Orntoft T, Sorensen K
Clin Cancer Res, 2014-04-15;20(8):2169-81. 2014-04-15
The secreted protein ANGPTL2 promotes metastasis of osteosarcoma cells through integrin alpha5beta1, p38 MAPK, and matrix metalloproteinases.
Authors: Odagiri H, Kadomatsu T, Endo M, Masuda T, Morioka M, Fukuhara S, Miyamoto T, Kobayashi E, Miyata K, Aoi J, Horiguchi H, Nishimura N, Terada K, Yakushiji T, Manabe I, Mochizuki N, Mizuta H, Oike Y
Sci Signal, 2014-01-21;7(309):ra7. 2014-01-21
MicroRNA-433 inhibits liver cancer cell migration by repressing the protein expression and function of cAMP response element-binding protein.
Authors: Yang Z, Tsuchiya H, Zhang Y, Hartnett M, Wang L
J Biol Chem, 2013-08-26;288(40):28893-9. 2013-08-26
Intermedin/adrenomedullin 2 is associated with implantation and placentation via trophoblast invasion in human pregnancy.
Authors: Havemann D, Balakrishnan M, Borahay M, Theiler R, Jennings K, Endsley J, Phelps J, Hankins G, Yallampalli C, Chauhan M
J Clin Endocrinol Metab, 2013-01-21;98(2):695-703. 2013-01-21
MDA-9/syntenin and IGFBP-2 promote angiogenesis in human melanoma.
Authors: Das S, Bhutia S, Azab B, Kegelman T, Peachy L, Santhekadur P, Dasgupta S, Dash R, Dent P, Grant S, Emdad L, Pellecchia M, Sarkar D, Fisher P
Cancer Res, 2012-12-10;73(2):844-54. 2012-12-10
Myofibroblasts in interstitial lung diseases show diverse electron microscopic and invasive features.
Authors: Karvonen H, Lehtonen S, Sormunen R, Harju T, Lappi-Blanco E, Bloigu R, Kaarteenaho R
Lab Invest, 2012-06-18;92(9):1270-84. 2012-06-18
Protein kinase C iota as a therapeutic target in alveolar rhabdomyosarcoma.
Authors: Kikuchi K, Soundararajan A, Zarzabal L, Weems C, Nelon L, Hampton S, Michalek J, Rubin B, Fields A, Keller C
Oncogene, 2012-02-20;32(3):286-95. 2012-02-20
Tumor cell-derived angiopoietin-like protein ANGPTL2 is a critical driver of metastasis.
Authors: Endo M, Nakano M, Kadomatsu T, Fukuhara S, Kuroda H, Mikami S, Hato T, Aoi J, Horiguchi H, Miyata K, Odagiri H, Masuda T, Harada M, Horio H, Hishima T, Nomori H, Ito T, Yamamoto Y, Minami T, Okada S, Takahashi T, Mochizuki N, Iwase H, Oike Y
Cancer Res, 2012-02-16;72(7):1784-94. 2012-02-16
Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer.
Authors: Zheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, Edil BH, Mizuma M, Sharma R, Le DT, Anders RA, Illei PB, Van Eyk JE, Maitra A, Laheru D, Jaffee EM
PLoS ONE, 2011-04-29;6(4):e19390. 2011-04-29
Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma.
Authors: Jiang L, Liu X, Kolokythas A, Yu J, Wang A, Heidbreder CE, Shi F, Zhou X
Int. J. Cancer, 2010-08-01;127(3):505-12. 2010-08-01
Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13.
Authors: Nurmenniemi S, Kuvaja P, Lehtonen S
Exp. Cell Res., 2010-05-27;316(16):2676-82. 2010-05-27
For the 96-well Cultrex Basement Membrane Extract Cell Invasion/Migration Assays, can a partial plate be used, and the rest saved for later?
The 96-well microplate chamber does not have removable strips. However, the plate is stable, so unused wells may be used at a later date. The difficult part is to maintain sterility. To prevent contamination, unused wells may be sealed using a sterile sealing film. The BME coating needs to be applied fresh before each invasion assay. The BME and Calcein AM may be divided into working aliquots to limit freeze-thaws and stored at the temperature recommended in the kit booklet, for optimal stability. For maximum flexibility, we would recommend using the 24-well assays, as inserts for the 24-well plate are removable.
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