Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well

Assesses cell invasion through a complex extracellular matrix
Catalog # Availability Size / Price Qty
3455-096-K
Migration of NIH-3T3 and HT1080 Cells_Cultrex Cell Invasion_3455-096-K
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Citations (30)
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Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well Summary

Provides a tool for assessing cell invasion through a complex extracellular matrix.

Key Benefits

• Standardized assay for extracellular matrix invasion
• Available in 24 and 96 well formats
• Allows optimization for specific cell lines

Why Use Cultrex BME Cell Invasion Assay, 96-well?

The Cultrex BME Cell Invasion Assays provide systems where the researcher can determine the optimal BME coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through BME may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Kit Contents

• 5X Cultrex Basement Membrane Extract (BME) Coating Solution

  • 10X Coating Buffer
  • Calcein AM
  • Cell Invasion Inserts
  • 25X Cell Wash Buffer
  • 10X Cell Dissociation Solution
  • Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
  • Specifications

    Testing Cell Culture
    Tested for the ability to support cell attachment and spreading of HT1080 human fibrosarcoma cells.

    Tested to support EMT-induced cell invasion and migration using A549 human lung carcinoma cell lines.
    Shipping Conditions
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
    Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
    Species
    Multi-species

    Limitations

    For research use only. Not for diagnositic use.

    Product Datasheets

    Data Example

    FBS Stimulates Migration of NIH-3T3 and HT1080 Cells.  The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K3456-096-K3457-096-K3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.

    Citations for Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    30 Citations: Showing 1 - 10
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    1. Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1's pro-metastatic functions in esophageal cancer
      Authors: G Li, HW Qi, HG Dong, P Bai, M Sun, HY Liu
      Cancer Cell Int., 2020;20(0):355.  2020
    2. Phosphorylated Rasal2 facilitates breast cancer progression
      Authors: X Wang, C Qian, Y Yang, MY Liu, Y Ke, ZM Qian
      EBioMedicine, 2019;0(0):.  2019
    3. MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
      Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
      Sci Rep, 2019;9(1):9776.  2019
    4. Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells
      Authors: M Paku?a, A Witucka, P Uruski, A Radziemski, R Moszy?ski, D Szpurek, K Maksin, A Wo?niak, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
      Sci Rep, 2019;9(1):7587.  2019
    5. PYK2 promotes HER2-positive breast cancer invasion
      Authors: SI Al-Juboori, J Vadakekola, S Idri, S Wagner, D Zafeiris, JR Pearson, R Almshayakh, M Caraglia, V Desiderio, AK Miles, DJ Boocock, GR Ball, T Regad
      J. Exp. Clin. Cancer Res., 2019;38(1):210.  2019
    6. The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
      Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
      Int J Mol Sci, 2019;20(1):.  2019
    7. Thyroid cancer stem-like cell exosomes: regulation of EMT via transfer of lncRNAs
      Authors: H Hardin, H Helein, K Meyer, S Robertson, R Zhang, W Zhong, RV Lloyd
      Lab. Invest., 2018;98(9):1133-1142.  2018
    8. Antineoplastic effects of selective CDK9 inhibition with atuveciclib on cancer stem-like cells in triple-negative breast cancer
      Authors: D Brisard, F Eckerdt, LA Marsh, GT Blyth, S Jain, M Cristofani, D Horiuchi, LC Platanias
      Oncotarget, 2018;9(99):37305-37318.  2018
    9. Metformin inhibits gastric cancer cells metastatic traits through suppression of epithelial-mesenchymal transition in a glucose-independent manner
      Authors: S Valaee, MM Yaghoobi, M Shamsara
      PLoS ONE, 2017;12(3):e0174486.  2017
    10. MicroRNA-182 targets protein phosphatase 1 regulatory inhibitor subunit 1C in glioblastoma
      Authors: L Liu, X Zhang, C Nan, Z Zhao, S Ma, W Li, H Hu, Z Liang
      Oncotarget, 2017;8(70):114677-114684.  2017
    11. Heterogeneous stromal signaling within the tumor microenvironment controls the metastasis of pancreatic cancer
      Cancer Res., 2016;0(0):.  2016
    12. Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells
      Sci Rep, 2016;6(0):33132.  2016
    13. The roles of the epithelial-mesenchymal transition marker PRRX1 and miR-146b-5p in papillary thyroid carcinoma progression.
      Authors: Hardin H, Guo Z, Shan W, Montemayor-Garcia C, Asioli S, Yu X, Harrison A, Chen H, Lloyd R
      Am J Pathol, 2014;184(8):2342-54.  2014
    14. Protein kinase C iota as a therapeutic target in alveolar rhabdomyosarcoma.
      Authors: Kikuchi K, Soundararajan A, Zarzabal L, Weems C, Nelon L, Hampton S, Michalek J, Rubin B, Fields A, Keller C
      Oncogene, 2013;32(3):286-95.  2013
    15. Myofibroblasts in interstitial lung diseases show diverse electron microscopic and invasive features.
      Authors: Karvonen H, Lehtonen S, Sormunen R, Harju T, Lappi-Blanco E, Bloigu R, Kaarteenaho R
      Lab Invest, 2012;92(9):1270-84.  2012
    16. Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer.
      Authors: Zheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, Edil BH, Mizuma M, Sharma R, Le DT, Anders RA, Illei PB, Van Eyk JE, Maitra A, Laheru D, Jaffee EM
      PLoS ONE, 2011;6(4):e19390.  2011
    17. Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma.
      Authors: Jiang L, Liu X, Kolokythas A, Yu J, Wang A, Heidbreder CE, Shi F, Zhou X
      Int. J. Cancer, 2010;127(3):505-12.  2010
    18. Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13.
      Authors: Nurmenniemi S, Kuvaja P, Lehtonen S
      Exp. Cell Res., 2010;316(16):2676-82.  2010
    19. The MCT4 Gene: A Novel, Potential Target for Therapy of Advanced Prostate Cancer.
      Authors: Choi S, Xue H, Wu R, Fazli L, Lin D, Collins C, Gleave M, Gout P, Wang Y
      Clin Cancer Res, 0;22(11):2721-33.  0
    20. Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes.
      Authors: Smith G, Howell G, Phillips C, Muench S, Ponnambalam S, Harrison M
      J Biol Chem, 0;291(16):8500-15.  0
    21. 1alpha,25-Dihydroxyvitamin D3 Inhibits Esophageal Squamous Cell Carcinoma Progression by Reducing IL6 Signaling.
      Authors: Chen P, Hsieh C, Wu C, Yen T, Lin P, Chen W, Chen M
      Mol Cancer Ther, 0;14(6):1365-75.  0
    22. Hypermethylation of the GABRE~miR-452~miR-224 promoter in prostate cancer predicts biochemical recurrence after radical prostatectomy.
      Authors: Kristensen H, Haldrup C, Strand S, Mundbjerg K, Mortensen M, Thorsen K, Ostenfeld M, Wild P, Arsov C, Goering W, Visakorpi T, Egevad L, Lindberg J, Gronberg H, Hoyer S, Borre M, Orntoft T, Sorensen K
      Clin Cancer Res, 0;20(8):2169-81.  0
    23. Intermedin/adrenomedullin 2 is associated with implantation and placentation via trophoblast invasion in human pregnancy.
      Authors: Havemann D, Balakrishnan M, Borahay M, Theiler R, Jennings K, Endsley J, Phelps J, Hankins G, Yallampalli C, Chauhan M
      J Clin Endocrinol Metab, 0;98(2):695-703.  0
    24. MDA-9/syntenin and IGFBP-2 promote angiogenesis in human melanoma.
      Authors: Das S, Bhutia S, Azab B, Kegelman T, Peachy L, Santhekadur P, Dasgupta S, Dash R, Dent P, Grant S, Emdad L, Pellecchia M, Sarkar D, Fisher P
      Cancer Res, 0;73(2):844-54.  0
    25. Tumor cell-derived angiopoietin-like protein ANGPTL2 is a critical driver of metastasis.
      Authors: Endo M, Nakano M, Kadomatsu T, Fukuhara S, Kuroda H, Mikami S, Hato T, Aoi J, Horiguchi H, Miyata K, Odagiri H, Masuda T, Harada M, Horio H, Hishima T, Nomori H, Ito T, Yamamoto Y, Minami T, Okada S, Takahashi T, Mochizuki N, Iwase H, Oike Y
      Cancer Res, 0;72(7):1784-94.  0
    26. Semaphorin 3D autocrine signaling mediates the metastatic role of annexin A2 in pancreatic cancer.
      Authors: Foley K, Rucki A, Xiao Q, Zhou D, Leubner A, Mo G, Kleponis J, Wu A, Sharma R, Jiang Q, Anders R, Iacobuzio-Donahue C, Hajjar K, Maitra A, Jaffee E, Zheng L
      Sci Signal, 0;8(388):ra77.  0
    27. Reciprocal regulation of amino acid import and epigenetic state through Lat1 and EZH2.
      Authors: Dann S, Ryskin M, Barsotti A, Golas J, Shi C, Miranda M, Hosselet C, Lemon L, Lucas J, Karnoub M, Wang F, Myers J, Garza S, Follettie M, Geles K, Klippel A, Rollins R, Fantin V
      EMBO J, 0;34(13):1773-85.  0
    28. MicroRNA-433 inhibits liver cancer cell migration by repressing the protein expression and function of cAMP response element-binding protein.
      Authors: Yang Z, Tsuchiya H, Zhang Y, Hartnett M, Wang L
      J Biol Chem, 0;288(40):28893-9.  0
    29. The secreted protein ANGPTL2 promotes metastasis of osteosarcoma cells through integrin alpha5beta1, p38 MAPK, and matrix metalloproteinases.
      Authors: Odagiri H, Kadomatsu T, Endo M, Masuda T, Morioka M, Fukuhara S, Miyamoto T, Kobayashi E, Miyata K, Aoi J, Horiguchi H, Nishimura N, Terada K, Yakushiji T, Manabe I, Mochizuki N, Mizuta H, Oike Y
      Sci Signal, 0;7(309):ra7.  0
    30. Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels.
      Authors: Fujimura K, Choi S, Wyse M, Strnadel J, Wright T, Klemke R
      J Biol Chem, 0;290(50):29907-19.  0

    FAQs

    1. For the 96-well Cultrex Basement Membrane Extract Cell Invasion/Migration Assays, can a partial plate be used, and the rest saved for later?

      • The 96-well microplate chamber does not have removable strips. However, the plate is stable, so unused wells may be used at a later date. The difficult part is to maintain sterility. To prevent contamination, unused wells may be sealed using a sterile sealing film. The BME coating needs to be applied fresh before each invasion assay. The BME and Calcein AM may be divided into working aliquots to limit freeze-thaws and stored at the temperature recommended in the kit booklet, for optimal stability. For maximum flexibility, we would recommend using the 24-well assays, as inserts for the 24-well plate are removable.

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