Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well
Cultrex Basement Membrane Extract Cell Invasion Assay, 96-well Summary
• Standardized assay for extracellular matrix invasion
• Available in 24 and 96 well formats
• Allows optimization for specific cell lines
Why Use Cultrex BME Cell Invasion Assay, 96-well?
The Cultrex BME Cell Invasion Assays provide systems where the researcher can determine the optimal BME coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through BME may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.
Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
• 5X Cultrex Basement Membrane Extract (BME) Coating Solution
• 10X Coating Buffer
• Calcein AM
• Cell Invasion Inserts
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution
• Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Tested to support EMT-induced cell invasion and migration using A549 human lung carcinoma cell lines.
For research use only. Not for diagnositic use.
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells. The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.
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