Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Cultrex Collagen IV Cell Invasion Assay
R&D Systems | Catalog # 3458-096-K
Asseses cell invasion through Collagen IV
Discontinued Product
3458-096-K has been discontinued.
View all Collagen IV products.
Key Product Details
Features
The Cultrex Collagen IV Cell Invasion Assay provides a tool for assessing cell invasion through Collagen IV.
Species
Mouse
Product Summary for Cultrex Collagen IV Cell Invasion Assay
Why Use Cultrex Collagen IV Cell Invasion Assay?
The Cultrex Collagen IV Cell Invasion Assays provide systems where the researcher can determine the optimal Collagen IV coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through Collagen IV may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex Collagen IV Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.
Cultrex Mouse Collagen IV is purified from Engelbreth-Holm-Swarm (EHS) tumor. Collagen IV is a component of the basement membrane which is a continuous sheet of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.
Cultrex Mouse Collagen IV is purified from Engelbreth-Holm-Swarm (EHS) tumor. Collagen IV is a component of the basement membrane which is a continuous sheet of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.
Loading...
Product Specifications
Cell Culture Testing
Tested to support EMT-induced cell invasion using A549 human lung carcinoma cell lines.
Scientific Data Images for Cultrex Collagen IV Cell Invasion Assay
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells.
The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.Kit Contents for Cultrex Collagen IV Cell Invasion Assay
- 10X Coating Buffer
- 5X Collagen IV Solution
- Calcein AM
- Cell Invasion Chamber
- 25X Cell Wash Buffer
- 10X Cell Dissociation Solution
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Collagen IV
Alternate Names
BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
Additional Collagen IV Products
Product Documents for Cultrex Collagen IV Cell Invasion Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for Cultrex Collagen IV Cell Invasion Assay
Powered by Bioz
Customer Reviews for Cultrex Collagen IV Cell Invasion Assay
There are currently no reviews for this product. Be the first to review Cultrex Collagen IV Cell Invasion Assay and earn rewards!
Have you used Cultrex Collagen IV Cell Invasion Assay?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
FAQs for Cultrex Collagen IV Cell Invasion Assay
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: The kit comes with both black and clear microplates. Do I need to use both?
A: The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired. The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top. The clear assay plate provides compatibility for plate readers that read from the bottom. Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).
