Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).
Cultrex Laminin I Cell Invasion Assay, 96-well
R&D Systems | Catalog # 3456-096-K
Key Product Details
Features
Key Benefits
- Standardized assay for Laminin I cell invasion
- Available in 24 and 96 well formats
- Allows optimization for specific cell lines
Species
Product Summary for Cultrex Laminin I Cell Invasion Assay, 96-well
Cultrex Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.
Product Specifications
Cell Culture Testing
Scientific Data Images for Cultrex Laminin I Cell Invasion Assay, 96-well
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells.
The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.Kit Contents for Cultrex Laminin I Cell Invasion Assay, 96-well
- 10X Coating Buffer
- 5X Laminin I Solution
- Calcein AM
- Cell Invasion Chamber
- 25X Cell Wash Buffer
- 10X Cell Dissociation Solution
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
Storage
Background: Laminin-1
Alternate Names
Additional Laminin-1 Products
Product Documents for Cultrex Laminin I Cell Invasion Assay, 96-well
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
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FAQs for Cultrex Laminin I Cell Invasion Assay, 96-well
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Q: The kit comes with both black and clear microplates. Do I need to use both?
A: The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired. The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top. The clear assay plate provides compatibility for plate readers that read from the bottom. Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).