CXCL11/I-TAC Antibody (10C6) - Azide and BSA Free

Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Analysis of 0.25 ug of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel.

ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Human ITAC was performed by coating a blank 96-well microtiter plate with 100ul per well of Human ITAC monoclonal antibody in duplicate at 1, 3, 4, 5, 7, and 9ug/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300ul per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50ul per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50ul of Human ITAC biotinylated monoclonal antibody in all applicable wells at 0.2ug/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100ul per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark.