CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

Novus Biologicals | Catalog # NBP1-43338

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E8-C7-F10

Format

BSA Free
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD182.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

Immunohistochemistry-Paraffin: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Immunohistochemistry-Paraffin: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Immunohistochemistry-Paraffin: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - CXCR2positive breast cancer cells in the human brain. CXCR2-red fluorochrome, nucleus-DAPI. Antibody used at dilution of 1:100. This image was submitted via customer Review.
Flow Cytometry: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Flow Cytometry: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Analysis using the PE conjugate of NBP1-43338. Staining of normal human peripheral blood cells with Anti-Human CD4 APC (left) or Anti-Human CD56 (NCAM) APC (right) and Anti-Human CD182 (CXCR2) PE. Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Flow Cytometry: CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free [NBP1-43338]

Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD182 (CXCR2) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE. Cells in the large scatter population were used for analysis.

Applications for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Reviewed Applications

Read 1 review rated 5 using NBP1-43338 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CXCR2/IL-8RB

The 5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of its ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Gene Symbol

CXCR2

Additional CXCR2/IL-8RB Products

Product Documents for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CXCR2/IL-8 RB Antibody (5E8-C7-F10)
    Name: Ilya Ulasov
    Application: Immunohistochemistry-Paraffin
    Sample Tested: breast cancer in the human brain ( metastases)
    Species: Human
    Verified Customer | Posted 02/22/2017
    CXCR2positive breast cancer cells in the human brain. CXCR2-red fluorochrome, nucleus-DAPI
    standard protocol, dilution 1:100
    CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free NBP1-43338

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies