CXCR4 Antibody (DMC680) - Chimeric

Novus Biologicals | Catalog # NBP3-48380

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC680
View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

CXCR4 recombinant protein expressed in mammalian cells (Accession# P61073; A0A0U3FJG0; A0A0U3GXA9). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CXCR4 Antibody (DMC680) - Chimeric

CXCR4 Antibody (DMC680) - Chimeric

Flow Cytometry: CXCR4 Antibody (DMC680) - Chimeric [NBP3-48380]

Flow cytometry analysis of antigen binding of anti-human CXCR4 mAb(NBP3-48380). (A) NBP3-48380 does not bind to hepG2 cells that do not express CXCR4. (B) A clear peak shift of NBP3-48380 was seen compared to the control when incubated with CXCR4-expressing Hela cells, indicating strong binding of NBP3-48380 to CXCR4. Antibodies were incubated at 5 ug/mL.
CXCR4 Antibody (DMC680) - Chimeric

Flow Cytometry: CXCR4 Antibody (DMC680) - Chimeric [NBP3-48380]

CXCR4 protein is expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with Anti-CXCR4 (DMC680) on HEK293 cells transfected with human CXCR4 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Applications for CXCR4 Antibody (DMC680) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR4

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lympho- and myelopoiesis, cardiogenesis, blood vessel formation and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2. The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Gene Symbol

CXCR4

Additional CXCR4 Products

Product Documents for CXCR4 Antibody (DMC680) - Chimeric

Certificate of Analysis

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Product Specific Notices for CXCR4 Antibody (DMC680) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CXCR4 Antibody (DMC680) - Chimeric

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  • Q: Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

    A:

    CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

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