CYB5R3 Antibody (OTI2A10)

Novus Biologicals | Catalog # NBP2-02705

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2A10
View all CYB5R3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYB5R3 Antibody (OTI2A10)

Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.
Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.
Western Blot: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Western Blot: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (OTI2A10) [NBP2-02705]

Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - Analysis of Jurkat cells, using anti-CYB5R3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for CYB5R3 Antibody (OTI2A10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.76 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYB5R3

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq].

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Entrez Gene IDs

1727 (Human)

Gene Symbol

CYB5R3

Additional CYB5R3 Products

Product Documents for CYB5R3 Antibody (OTI2A10)

Certificate of Analysis

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Product Specific Notices for CYB5R3 Antibody (OTI2A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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