Cyclin D3 Antibody (4C5C8)

Novus Biologicals | Catalog # NBP3-16309

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4C5C8 expressed in HEK293
View all Cyclin D3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 202-292 of human Cyclin D3 (P30281). MIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin D3 Antibody (4C5C8)

Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]

Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]

Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]

Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]

Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Cyclin D3 Antibody (4C5C8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin D3

During the G1 phase of the cell cycle, a cell must choose between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.

Alternate Names

CCND3

Gene Symbol

CCND3

Additional Cyclin D3 Products

Product Documents for Cyclin D3 Antibody (4C5C8)

Certificate of Analysis

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Product Specific Notices for Cyclin D3 Antibody (4C5C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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