Cyclin E1 [p Thr395] Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05378
![Western Blot: Cyclin E1 [p Thr395] AntibodyBSA Free [NBP3-05378]](https://resources.rndsystems.com/images/products/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] AntibodyBSA Free [NBP3-05378]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1). LLTPP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Cyclin E1 [p Thr395] Antibody - BSA Free (NBP3-05378) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cyclin E1 [p Thr395] Antibody - BSA Free
Western Blot: Cyclin E1 [p Thr395] AntibodyBSA Free [NBP3-05378]
Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.![Cyclin E1 [p Thr395] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg "Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -")
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Cyclin E1 [p Thr395] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg "Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -")
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Cyclin E1 [p Thr395] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg "Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -")
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] -
Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Cyclin E1 [p Thr395] Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin E1
Alternate Names
CCNE, CCNE1
Gene Symbol
CCNE1
Additional Cyclin E1 Products
Product Documents for Cyclin E1 [p Thr395] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cyclin E1 [p Thr395] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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