Cystatin B/Stefin B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31838

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chimpanzee (98%), Rhesus Macaque (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cystatin B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cystatin B/Stefin B. The exact sequence is proprietary.

Reactivity Notes

Pig (81%).

Localization

Nucleus/Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cystatin B/Stefin B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunocytochemistry/Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraformaldehyde-fixed A431, using Cystatin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraffin-embedded tonsil. Cystatin B antibody dilution: 1:100.
Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraffin-embedded mouse brain. Cystatin B antibody diluted at 1:100.

Applications for Cystatin B/Stefin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cystatin B

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Alternate Names

CSTB, Stefin B

Gene Symbol

CSTB

Additional Cystatin B Products

Product Documents for Cystatin B/Stefin B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cystatin B/Stefin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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FAQs

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