Cystatin S Antibody (OTI2H10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70481

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2H10

Format

Azide and BSA Free
View all Cystatin S Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CST4(NP_001890) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cystatin S Antibody (OTI2H10) - Azide and BSA Free

Western Blot: Cystatin S Antibody (OTI2H10)Azide and BSA Free [NBP2-70481]

Western Blot: Cystatin S Antibody (OTI2H10)Azide and BSA Free [NBP2-70481]

Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cystatin S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cystatin S.
Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Immunocytochemistry/Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cystatin S.
Flow Cytometry: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Flow Cytometry: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Flow Cytometry: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cystatin S antibody, and then analyzed by flow cytometry.
Western Blot: Cystatin S Antibody (OTI2H10)Azide and BSA Free [NBP2-70481]

Western Blot: Cystatin S Antibody (OTI2H10)Azide and BSA Free [NBP2-70481]

Western Blot: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Analysis of extracts (10ug) from HeLa cell line by using anti-CST4 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Immunocytochemistry/ Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481]

Immunocytochemistry/Immunofluorescence: Cystatin S Antibody (OTI2H10) - Azide and BSA Free [NBP2-70481] - Staining of HeLa cells using anti-Cystatin S mouse monoclonal antibody.

Applications for Cystatin S Antibody (OTI2H10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin S

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq]

Alternate Names

CST4

Gene Symbol

CST4

Additional Cystatin S Products

Product Documents for Cystatin S Antibody (OTI2H10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cystatin S Antibody (OTI2H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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FAQs

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