Cytochrome c Antibody (6H2.B4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80687

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H2.B4

Format

Azide and BSA Free
View all Cytochrome c Primary Antibodies »

Product Specifications

Immunogen

Rat cytochrome c was used as immunogen to generate 6H2.B4 hybridoma clone (Goshorn et al, 1991). The antibody recognizes an epitope around residue 62 of rat Cytochrome c, based on competitive ELISA results (Goshorn et al. 1991).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cytochrome c Antibody (6H2.B4) - Azide and BSA Free

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12. Image from the standard format of this antibody.
Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687]

Immunoprecipitation: Cytochrome c Antibody (6H2.B4) - Azide and BSA Free [NBP2-80687] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents. Image from the standard format of this antibody.

Applications for Cytochrome c Antibody (6H2.B4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 16540654)

Immunohistochemistry

reported in scientific literature (PMID 28194435)

Immunoprecipitation

2 ug/1X10^6 cells

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome c

In addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Alternate Names

CYCS

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (6H2.B4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytochrome c Antibody (6H2.B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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