Cytohesin 2 Antibody (OTI4D9)

Novus Biologicals | Catalog # NBP3-44710

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D9
View all Cytohesin 2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYTH2 (NP_059431) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytohesin 2 Antibody (OTI4D9)

Cytohesin 2 Antibody (OTI4D9) Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYTH2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Cytohesin 2 Antibody (OTI4D9) Western Blot;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Western Blot;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY CYTH2 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYTH2 (1:2000). Positive lysates
Cytohesin 2 Antibody (OTI4D9) Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYTH2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Cytohesin 2 Antibody (OTI4D9) Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemistry;Cytohesin 2 Antibody (OTI4D9)[NBP3-44710]-

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYTH2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Applications for Cytohesin 2 Antibody (OTI4D9)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytohesin 2

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with ARF1, ARF3, and ARF6. PSCD2 protein is 83% homologous to PSCD1. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Cytohesin-2

Alternate Names

ARF exchange factor, ARNO, CYTH2, Protein ARNO, PSCD2, PSCD2L

Gene Symbol

CYTH2

Additional Cytohesin 2 Products

Product Documents for Cytohesin 2 Antibody (OTI4D9)

Certificate of Analysis

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Product Specific Notices for Cytohesin 2 Antibody (OTI4D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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