Cytokeratin 10 Antibody (MSVA-610M)

Novus Biologicals | Catalog # NBP3-57222

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-610M
View all Cytokeratin 10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-lenth human KRT10 protein. Swissprot P13645

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining..

Negative Control
Negitve control:Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytokeratin 10 Antibody (MSVA-610M)

Cytokeratin 10 Antibody (MSVA-610M)

Immunohistochemistry-Paraffin:Cytokeratin 10 Antibody (MSVA-610M)NBP3-57222

Strong Cytokeratin 10 immunostaining in a squamous cell carcinoma of the vulva. The basal cell layers are largely unstained.
Cytokeratin 10 Antibody (MSVA-610M)

Immunohistochemistry-Paraffin:Cytokeratin 10 Antibody (MSVA-610M)NBP3-57222

skin hairfollicel and sebaceous glands Zusatztext 2
Cytokeratin 10 Antibody (MSVA-610M)

Immunohistochemistry-Paraffin:Cytokeratin 10 Antibody (MSVA-610M)NBP3-57222

Absence of KRT10 staining in non keratinizing squamous epithelium of the esophagus.

Applications for Cytokeratin 10 Antibody (MSVA-610M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 10

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Alternate Names

BCIE, CK-10, CK10, EHK2A, EHK2B, K10, KPP

Gene Symbol

KRT1, KRT10

Additional Cytokeratin 10 Products

Product Documents for Cytokeratin 10 Antibody (MSVA-610M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 10 Antibody (MSVA-610M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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