Cytokeratin 14 Antibody (101) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89198

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 101 expressed in HEK293

Format

Azide and BSA Free
View all Cytokeratin 14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, a synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytokeratin 14 Antibody (101) - Azide and BSA Free

Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198]

Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198]

Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Immunochemical staining of human Cytokeratin14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000).
Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198]

Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody:Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size: 52 kDa

Applications for Cytokeratin 14 Antibody (101) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10000-1:20000

Immunohistochemistry-Paraffin

1:10000-1:20000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (101) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 14 Antibody (101) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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