Cytokeratin 14 Antibody (LL002)

Novus Biologicals | Catalog # NBP2-34270

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # LL002
View all Cytokeratin 14 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14. (Uniprot: P02533)

Localization

Cytoplasmic

Marker

Squamous Cell Marker

Specificity

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34675)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 14 Antibody (LL002)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270]

Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.
Cytokeratin 14 Antibody (LL002)

Western Blot: Cytokeratin 14 Antibody (LL002) [NBP2-34270] -

4-AP accelerated epithelization and keratinocytes terminal differentiation following skin burn.A, B Representative images and quantitative results of IF staining of keratinocyte markers (K10; terminal differentiation and K14; proliferative) in saline and 4-AP treated skin burn tissues on days 3 and 7. Scale bar = 100 um. n = 4 skin tissues per group. C, D Western blotting and (E) qRT-PCR quantitative results show that 4-AP treatment significantly increased keratin markers protein and gene (K10 and K14) expressions compared to the saline group on days 3 and 7. n = 3 skin tissues per group. Data are represented as mean +/- SEM. The statistical significance is indicated by asterisks (*P < 0.05, **P < 0.0021, and ***P < 0.0002 vs. saline group) and compared using two-tailed, unpaired t-tests. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39366954), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Cytokeratin 14 Antibody (LL002)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in A431 lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 56 kDa

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

UniProt

P02533 (Human)

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (LL002)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 14 Antibody (LL002)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 14 Antibody (LL002)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cytokeratin 14 Antibody (LL002)

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  • Q: I received this antibody today and the data sheet recommends a concentration of 1-2ug/ml for IF however nowhere is the stock concentration listed so I don't know what dilution to do. Data sheet says check label and the label does not have the conc. The LOT number I received is 3861-1P150129 Please let me know what concentration the stock is.

    A: I can confirm you that the the concentration of Lot# 3861-1P150129 of Cytokeratin 14 Antibody (LL002) [NBP2-34270] is 200ug/ml. This format of the antibody also contains 0.05% BSA and 0.05% Azide as stabalizer and preservative respectively. So you can try this antibody at 1:100 - 1:200 dilution range, and based on your pilot results on your particular samples, you can go for lower or higher dilution for obtaining the best signal.

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