Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43990

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9390

Format

Azide and BSA Free
View all Cytokeratin 20 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P35900, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free

Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Analysis in human rectum and prostate tissues using NBP3-43990 antibody. Corresponding Cytokeratin 20 RNA-seq data are presented for the same tissues.
Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Staining of human kidney shows no positivity in cells in tubules as expected.
Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Staining of human prostate shows no positivity in glandular cells as expected.
Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Staining of human rectum shows strong membranous / cytoplasmic positivity in glandular cells.
Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP3-43990]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 20 Antibody (CL9390) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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