Cytokeratin 7 Antibody (R17-S)
Novus Biologicals | Catalog # NBP1-30152
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # R17-S
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Product Specifications
Immunogen
Peptide derived from N-terminal sequence of human cytokeratin 7. Antibody recognizes the epitope between Ala22 - Ser38.
Epitope
Ala22 - Ser38
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28832662).
Specificity
This antibody is specific for the N terminus o Cytokeratin 7
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Scientific Data Images for Cytokeratin 7 Antibody (R17-S)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0009.jpgImmunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0011.jpgImmunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Cytokeratin-7-Antibody-R17-S-Immunohistochemistry-NBP1-30152-img0006.jpgImmunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Pulmonary adenocarcinoma (A), ductal pancreatic adenocarcinoma (B) and ductal carcinoma of the breast (C) showing diffuse CK7 positivity. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 7 monospecific clonal antibody.Western Blot: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -
Western Blot: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Cytokeratin 7 Antibody (R17-S)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-200
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25076207).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytokeratin 7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (R17-S)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 7 Antibody (R17-S)
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Cytokeratin 7 Antibody (R17-S)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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