Cytokeratin 7 Antibody (R17-S)

Novus Biologicals | Catalog # NBP1-30152

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # R17-S
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Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human cytokeratin 7. Antibody recognizes the epitope between Ala22 - Ser38.

Epitope

Ala22 - Ser38

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28832662).

Specificity

This antibody is specific for the N terminus o Cytokeratin 7

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Cytokeratin 7 Antibody (R17-S)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0009.jpg
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Cytokeratin-7-Antibody-R17-S-Immunocytochemistry-Immunofluorescence-NBP1-30152-img0011.jpg
Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Cytokeratin-7-Antibody-R17-S-Immunohistochemistry-NBP1-30152-img0006.jpg
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Pulmonary adenocarcinoma (A), ductal pancreatic adenocarcinoma (B) and ductal carcinoma of the breast (C) showing diffuse CK7 positivity. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 7 monospecific clonal antibody.
Cytokeratin 7 Antibody (R17-S)

Western Blot: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -

Western Blot: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 7 Antibody (R17-S)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 7 Antibody (R17-S)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Collagen type I.The total amount of collagen type I quantified as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (A) is shown. The total amount of ITGB1 as percent of total area in both control (n = 5 tumors) & HBOT (n = 5 tumors) primary tumors (B). A representative immunofluorescence (IF) staining picture from a control (C) & HBOT (D) tumor is shown. Green represents stroma cells (stained with antibody to mouse integrin-beta 1) red represents collagen type I secreted by the stromal cells (stained with antibody to mouse collagen type I), & blue shows the nuclei stained with DAPI. Scale bar represents 100 μm. Representative IF pictures are also shown to illustrate the compostion/proportion of the tumor cells (red, stained with cytokeratin 7 antibody) & the stromal cells (green, stained with integrin beta 1 antibody) from the control (E) & and HBOT (F) tumors, respectively. Scale bar represents 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28832662), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Cytokeratin 7 Antibody (R17-S)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-200
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25076207).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris-HCl (pH 8.0) and 20mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 7

Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Alternate Names

CK-7, CK7, K2C7, K7

Entrez Gene IDs

3855 (Human)

Gene Symbol

KRT7

UniProt

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (R17-S)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 7 Antibody (R17-S)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 7 Antibody (R17-S)

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Protocols

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