Cytokeratin 8 Antibody (KRT8/6472R)

Novus Biologicals | Catalog # NBP3-48923

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT8/6472R expressed in CHO
View all Cytokeratin 8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human KRT8 protein (Uniprot: P05787)

Localization

Cytoplasm, Nucleoplasm, Nucleus, Nucleus matrix

Specificity

Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for Cytokeratin 8 Antibody (KRT8/6472R)

Cytokeratin 8 Antibody (KRT8/6472R)

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (KRT8/6472R) [NBP3-48923] -

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Cytokeratin 8 Antibody (KRT8/6472R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.
Cytokeratin 8 Antibody (KRT8/6472R)

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (KRT8/6472R) [NBP3-48923] -

Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 8 Antibody (KRT8/6472R).

Applications for Cytokeratin 8 Antibody (KRT8/6472R)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 8

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody (KRT8/6472R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody (KRT8/6472R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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