Cytokeratin, LMW Antibody (KRTL/1077)
Novus Biologicals | Catalog # NBP2-44373
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # KRTL/1077
Loading...
Product Specifications
Immunogen
Recombinant human Cytokeratin, LMW protein (Uniprot: Q7Z794)
Reactivity Notes
Shows broad species reactivity.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47798)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin, LMW Antibody (KRTL/1077)
![Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]](https://resources.rndsystems.com/images/products/Cytokeratin-LMW-Antibody-KRTL-1077-Immunohistochemistry-Paraffin-NBP2-44373-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]")
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373] - Rat Stomach with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).![Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]](https://resources.rndsystems.com/images/products/Cytokeratin-LMW-Antibody-KRTL-1077-Immunohistochemistry-Paraffin-NBP2-44373-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]")
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373] - Human Skin stained with Cytokeratin, LMW Monoclonal Antibody (KRTL/1077).![Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]](https://resources.rndsystems.com/images/products/Cytokeratin-LMW-Antibody-KRTL-1077-Immunohistochemistry-Paraffin-NBP2-44373-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]")
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373]
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) [NBP2-44373] - Rat Oviduct stained with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).Applications for Cytokeratin, LMW Antibody (KRTL/1077)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin, LMW
Alternate Names
Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
Gene Symbol
KRT77
UniProt
Additional Cytokeratin, LMW Products
Product Documents for Cytokeratin, LMW Antibody (KRTL/1077)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin, LMW Antibody (KRTL/1077)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Cytokeratin, LMW Antibody (KRTL/1077)
There are currently no reviews for this product. Be the first to review Cytokeratin, LMW Antibody (KRTL/1077) and earn rewards!
Have you used Cytokeratin, LMW Antibody (KRTL/1077)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...