Cytokeratin, LMW Antibody (rKRTL/8751)

Novus Biologicals | Catalog # NBP3-23655

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rKRTL/8751
View all Cytokeratin, LMW Primary Antibodies »

Product Specifications

Immunogen

Human epidermal keratin

Localization

Cytoplasm.

Marker

Epithelial Marker

Specificity

This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Cytokeratin, LMW Antibody (rKRTL/8751)

Cytokeratin, LMW Antibody (rKRTL/8751) Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] -

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] -

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] - Formalin-fixed, paraffin-embedded human placenta stained with Cytokeratin, LMW Antibody (rKRTL/8751). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Cytokeratin, LMW Antibody (rKRTL/8751)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry-Paraffin: 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, LMW

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (rKRTL/8751)

Certificate of Analysis

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Product Specific Notices for Cytokeratin, LMW Antibody (rKRTL/8751)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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