Cytomegalovirus Antibody (DDG9 and CCH2)

Novus Biologicals | Catalog # NBP1-22532

Novus Biologicals
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Key Product Details

Species Reactivity

Cytomegalovirus, Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG2a Kappa Clone # DDG9 and CCH2
View all Cytomegalovirus Primary Antibodies »

Product Specifications

Immunogen

BALB/C mice were immunized with cytomegalovirus infected cell lysate. This antibody is a cocktail of DDG9 and CCH2.

Localization

Nuclear

Specificity

This cocktail antibody reacts with an immediate early and early CMV antigens. The antibody stains infected cells giving a nuclear staining pattern with an immediate early and an early antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG2a Kappa

Scientific Data Images for Cytomegalovirus Antibody (DDG9 and CCH2)

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).
Immunohistochemistry: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - Human Placenta CMV. HIER sodium citrate 6.0 - 30 minutes 1:10 dilution. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).
Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532]

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded human salivary glands (CMV infected) using Cytomegalovirus antibody (clone DDG9 and CCH2).

Applications for Cytomegalovirus Antibody (DDG9 and CCH2)

Application
Recommended Usage

Immunohistochemistry

1:10-1:25

Immunohistochemistry-Paraffin

1:10-1:25
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-22532 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytomegalovirus

Human cytomegalovirus, also called HCMV or human herpesvirus 5, is a double-stranded DNA, enveloped virus of 230-250 kilobases (kb) that is a member of the Herpesviridae family and the subfamily Betaherpesvirinae (1,2). Other herpesviruses, which share the characteristic of lifelong latency following primary infection, include herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), varicella zoster virus (VSV), and Epstein-Barr virus (EBV) (1). Cytomegalovirus entry into cells is mediated by a trimeric complex of glycoproteins gH, gL, and gO and a pentamer made of proteins gH, gL, UL128, UL130, and UL131A (1,3). The trimer binds PDGFRalpha for entry into fibroblasts while the pentamer binds neuropilin-2 for entry into endothelial and epithelial cells (1,4). Additionally, the fusion protein glycoprotein B (gB) plays a pivotal role for cytomegalovirus entry into the host cell membrane (1,3).

Cytomegalovirus is a common virus, infecting ~60% of adults in developed countries and over 90% in developing countries, as indicated by presence of specific IgG antibodies (2-4). Although a majority of people infected remain asymptomatic, immunocompromised people, organ transplant recipients, and fetuses and newborns are more susceptible to HCMV-associated diseases (1-4). Annually, up to 2% of newborns are born with HCMV, making it the most common congenital infection (2-4). Cytomegalovirus is known to cause sensorineural hearing loss (SNHL), mental retardation, chorioretinitis, skin lesions, as well as end organ disease (3,5). There are a few CMV therapeutics approved by FDA including valganciclovir and letermovir; however, despite some benefits, there is the need for new antivirals and combination therapies (5). Potential new treatment options include monoclonal antibodies and sirtuins (5).

References

1. Connolly SA, Jardetzky TS, Longnecker R. The structural basis of herpesvirus entry. Nat Rev Microbiol. 2021;19(2):110-121. https://doi.org/10.1038/s41579-020-00448-w

2. Griffiths P, Reeves M. Pathogenesis of human cytomegalovirus in the immunocompromised host. Nat Rev Microbiol. 2021;19(12):759-773. https://doi.org/10.1038/s41579-021-00582-z

3. Krstanovic F, Britt WJ, Jonjic S, Brizic I. Cytomegalovirus Infection and Inflammation in Developing Brain. Viruses. 2021;13(6):1078. Published 2021 Jun 4. https://doi.org/10.3390/v13061078

4. Griffiths P, Baraniak I, Reeves M. The pathogenesis of human cytomegalovirus. J Pathol. 2015;235(2):288-297. https://doi.org/10.1002/path.4437

5. Acosta E, Bowlin T, Brooks J, et al. Advances in the Development of Therapeutics for Cytomegalovirus Infections. J Infect Dis. 2020;221(Suppl 1):S32-S44. https://doi.org/10.1093/infdis/jiz493

Alternate Names

CMV; HHV 5; HHV5; Human herpesvirus 5

Additional Cytomegalovirus Products

Product Documents for Cytomegalovirus Antibody (DDG9 and CCH2)

Certificate of Analysis

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Product Specific Notices for Cytomegalovirus Antibody (DDG9 and CCH2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytomegalovirus Antibody (DDG9 and CCH2) (2)

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  • Cytomegalovirus Antibody (DDG9 and CCH2)
    Name: Hans Snyder
    Application: Immunohistochemistry
    Sample Tested: 5um paraffin and Placental tissue
    Species: Human
    Verified Customer | Posted 07/07/2017
    Human Placenta CMV
    HIER sodium citrate 6.0 - 30 minutes 1:10 dilution
    Cytomegalovirus Antibody (DDG9 and CCH2) NBP1-22532
  • Cytomegalovirus Antibody (DDG9 and CCH2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Gingival cells and Human Oral Keratinocyte
    Species: Human
    Verified Customer | Posted 11/16/2016
    Keratinocytes Infected with CMV at Harvested at Day 6. 3 Samples were infected with CMV that express DDG9 and CCH2
    Cytomegalovirus Antibody (DDG9 and CCH2) NBP1-22532

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