Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89924

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 008 expressed in HEK293

Format

Azide and BSA Free
View all Cytosolic beta-Glucosidase/GBA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Cytosolic beta-Glucosidase/GBA3 (Accession#: NP_066024.1; Met1-Leu469).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (008) [NBP2-89924]

Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (008) [NBP2-89924]

Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (008) [NBP2-89924] - Analysis of Human GBA3 expression on Jurkat cells. The cells were treated, stained with purified anti-Human GBA3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cytosolic beta-Glucosidase/GBA3 Antibody (008) [NBP2-89924] -

Immunocytochemistry/Immunofluorescence: Cytosolic beta-Glucosidase/GBA3 Antibody (008) [NBP2-89924] - Analysis of Human GBA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89924 antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm.

Applications for Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytosolic beta-Glucosidase/GBA3

GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM]

Alternate Names

CBGL1, Cytosolic betaGlucosidase, GBA3, GLUC, KLrP

Gene Symbol

GBA3

Additional Cytosolic beta-Glucosidase/GBA3 Products

Product Documents for Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytosolic beta-Glucosidase/GBA3 Antibody (008) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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