Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89895

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007 expressed in HEK293

Format

Azide and BSA Free
View all Cytosolic Sulfotransferase 2B1/SULT2B1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Cytosolic Sulfotransferase 2B1/SULT2B1 (Accession#: NP_004596.2; Asp 2-Ser 365) .

Specificity

No cross-reactivity in ELISA with: Human SULT2A1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images

Western Blot: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895]

Western Blot: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895]

Western Blot: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895] - Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDa Observed band size:41 kDa
Immunoprecipitation: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895]

Immunoprecipitation: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895]

Immunoprecipitation: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (007) [NBP2-89895] - Lane A:0.5 mg MCF-7 Whole Cell Lysate 0.5 uL anti-SULT2B1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SULT2B1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size: 41 kDa

Applications

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytosolic Sulfotransferase 2B1/SULT2B1

Sulfotransferase family cytosolic 2B member 1 isoform b, also known as SULT2B1, is a member of Sulfotransferase family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT2B1b is localized in the cytosol and nuclei of human cells. SULT2B1b is selective for the sulfation of 3beta-hydroxysteroids such as dehydroepiandrosterone and pregnenolone, and may also have a role in cholesterol sulfation in human skin. Recombinant human SULT2B1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HSST2, SULT2B1

Gene Symbol

SULT2B1

Additional Cytosolic Sulfotransferase 2B1/SULT2B1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Sulfation Regulators

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