D4S234E Antibody

Name: D4S234E Antibody
Brand: Novus Biologicals
SKU: NBP2-26148
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-26148

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Human (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence EKGTKQPLLEDGFD-C corresponding to N-Terminus according to NP_077042.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for D4S234E Antibody

Western Blot: D4S234E Antibody [NBP2-26148]

Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: D4S234E Antibody [NBP2-26148]

Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP2-26148] - (3ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488).Right panel shows

Applications for D4S234E Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml

Application Notes

Western blot: Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Primary incubation was 1 hour.
Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: D4S234E

Alternate Names

carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein, D4S234Brain neuron cytoplasmic protein 1, DNA segment on chromosome 4 (unique) 234 expressed sequence, NEEP21, neuron-specific protein family member 1, NSG1, P21

Entrez Gene IDs

27065 (Human); 18196 (Mouse); 25247 (Rat)

Gene Symbol

NSG1

UniProt

NP_077042.2 (Rat)

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Certificate of Analysis

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Product Specific Notices for D4S234E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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