DAX1/NR0B1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32832

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Chimpanzee (99%), Rhesus Macaque (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DAX1/NR0B1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DAX1/NR0B1. The exact sequence is proprietary.

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAX1/NR0B1 Antibody - BSA Free

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832]

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832]

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832] - Cal27 xenograft, using NR0B1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
DAX1/NR0B1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] -

DAX1/NR0B1 antibody detects DAX1/NR0B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DAX1/NR0B1 protein stained by DAX1/NR0B1 antibody (NBP1-32832) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
DAX1/NR0B1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] -

NR0B1 antibody detects NR0B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR0B1 protein stained by NR0B1 antibody (NBP1-32832) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.

Applications for DAX1/NR0B1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:50
Application Notes
See Simple Western Antibody Database for Simple Western validation: Human fetal adrenal glands as sample; separated by size; antibody dilution of 1:50; observed molecular weight was ~51 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DAX1/NR0B1

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Long Name

Orphan Nuclear Receptor DAX1/Nuclear Receptor Subfamily 0, Group B, Member 1

Alternate Names

AHC, AHCH, NR0B1

Entrez Gene IDs

190 (Human)

Gene Symbol

NR0B1

UniProt

P51843 (Human)

Additional DAX1/NR0B1 Products

Product Documents for DAX1/NR0B1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DAX1/NR0B1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for DAX1/NR0B1 Antibody - BSA Free

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Protocols

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