DCAF4 Antibody

Novus Biologicals | Catalog # NBP1-05972

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WD repeat-containing protein 21A using the numbering given in entry NP_056419.2 (GeneID 26094).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCAF4 Antibody

Western Blot: DCAF4 Antibody [NBP1-05972]

Western Blot: DCAF4 Antibody [NBP1-05972]

Western Blot: DCAF4 Antibody [NBP1-05972] - Detection of Human and Mouse WDR21A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: DCAF4 Antibody [NBP1-05972]

Immunoprecipitation: DCAF4 Antibody [NBP1-05972]

Immunoprecipitation: DCAF4 Antibody [NBP1-05972] - Detection of human WDR21A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 (lot NBP1-05972-2) used for IP at 6 ug per reaction. WDR21A was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05972-1) and another rabbit anti-WDR21A antibody. For blotting immunoprecipitated WDR21A, NBP1-05972 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for DCAF4 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 µg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DCAF4

This gene encodes a WD repeat-containing protein. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Alternate Names

DDB1 and CUL4 associated factor 4, DDB1- and CUL4-associated factor 4, DKFZp434K114, MGC20547, MGC46524, WD repeat domain 21, WD repeat domain 21A, WD repeat-containing protein 21A, WDR21, WDR21A

Entrez Gene IDs

26094 (Human)

Gene Symbol

DCAF4

Additional DCAF4 Products

Product Documents for DCAF4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DCAF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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