DDIT4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85211

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDIT4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDIT4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-85211]

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-85211]

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-85211] - Staining of human cell line hTCEpi shows localization to cytosol.
Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP1-85211]

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP1-85211]

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP1-85211] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for DDIT4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDIT4

REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner.

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Gene Symbol

DDIT4

Additional DDIT4 Products

Product Documents for DDIT4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DDIT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDIT4 Antibody - BSA Free

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Protocols

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FAQs

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