DEC-205/CD205 Antibody (MG38) - BSA Free

Novus Biologicals | Catalog # NBP2-00328

Clone MG38 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MG38

Format

BSA Free
View all DEC-205/CD205 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is CD205 (DEC-205).

Reactivity Notes

Human

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for DEC-205/CD205 Antibody (MG38) - BSA Free

Flow Cytometry: DEC-205/CD205 Antibody (MG38) - BSA Free [NBP2-00328]

Flow Cytometry: DEC-205/CD205 Antibody (MG38) - BSA Free [NBP2-00328]

Flow Cytometry: CD205 Antibody (MG38) [NBP2-00328] - Staining of normal human peripheral blood cells (left) and mature dendritic cells (right) with Mouse IgG2b kappa Isotype Control Purified (open histogram) or 0.015 ug of Anti-Human CD205 Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Applications for DEC-205/CD205 Antibody (MG38) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 0.03 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The MG38 antibody has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DEC-205/CD205

The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.

Alternate Names

CD205, DEC205, Ly75

Gene Symbol

LY75

Additional DEC-205/CD205 Products

Product Documents for DEC-205/CD205 Antibody (MG38) - BSA Free

Certificate of Analysis

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Product Specific Notices for DEC-205/CD205 Antibody (MG38) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail