DEF6 Antibody

Novus Biologicals | Catalog # NB300-837

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DEF6 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence ALRKELLKSIWYA-C corresponding to N-Terminus according to NP_071330.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DEF6 Antibody

Western Blot: DEF6 Antibody [NB300-837]

Western Blot: DEF6 Antibody [NB300-837]

Western Blot: DEF6 Antibody [NB300-837] - Analysis of DEF6 on Human Thymus lysate using NB300-837 at 1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DEF6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human thymus lysates (calculated MW of 73.9 kDa band according to NP_071330.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Alternate Names

DEF-6, differentially expressed in FDCP 6 homolog, differentially expressed in FDCP 6 homolog (mouse), IBPdifferentially expressed in FDCP (mouse homolog) 6, IRF4-binding protein, SLAT, SWAP70L, SWAP-70-like adaptor protein of T cells

Entrez Gene IDs

50619 (Human); 23853 (Mouse)

Gene Symbol

DEF6

UniProt

NP_071330. (Human)

Additional DEF6 Products

Product Documents for DEF6 Antibody

Certificate of Analysis

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Product Specific Notices for DEF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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