DENND3 [p Thr450] Antibody - Azide Free

Novus Biologicals | Catalog # NBP3-11946

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all DENND3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 conjugated to KLH. (UniProt: D4A2H4)

Modification

p Thr450

Specificity

Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Thr450. The immunolabeling is completely eliminated by treatment with gamma-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DENND3 [p Thr450] Antibody - Azide Free

Western Blot: DENND3 [p Thr450] Antibody [NBP3-11946]

Western Blot: DENND3 [p Thr450] Antibody [NBP3-11946]

Western Blot: DENND3 [p Thr 450] Antibody [NBP3-11946] - Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Thr450 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (lambda-Ptase, 800 units/1mg protein for 30 min).

Applications for DENND3 [p Thr450] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: DENND3

Alternate Names

DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3

Gene Symbol

DENND3

Additional DENND3 Products

Product Documents for DENND3 [p Thr450] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for DENND3 [p Thr450] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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