Desmocollin-1 Antibody - BSA Free

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• Desmocollin-1 Antibody

  Name: Mickaël Pinto

  Application: Immunohistochemistry-Paraffin

  Sample Tested: Paraffin sections of canine folicular skin (dogs with pemphigus foliaceus)

  Species: Canine

  Verified Customer | Posted 11/07/2019

  Optical microscope (x20). IHC anti-desmocollin-1. Subcorneal epidermal pustule in dog with PF. Patchy staining pattern in some keratinocytes, in perilesional epidermis, as well as in acantholytic keratinocytes inside the lesion.

  ABSTRACT Background Pemphigus foliaceus is the most common autoimmune disease in dogs. It is characterized histologically by subcorneal pustules and acantholysis. Desmocollin-1 is the major target antigen of autoantibodies in dogs. Previous immunostaining studies have shown possible pathogenic similarities between canine and human pemphigus. Objetives To investigate the pathogenesis of acantholysis in canine Pemphigus Foliaceus, we decided to review the main histopathological findings and observe the variations of the labeling of desmocollin-1 by immunohistochemistry in skin biopsies from sick dogs. Material and methods Five cases of dogs with Pemphigus Foliaceus were selected and histopathological findings were reviewed by hematoxylin-eosin staining. Then the samples were stained following an immunohistochemical protocol, with an anti-desmocolin-1 antibody. Results The 5 cases presented typical histological characteristics of PFc: pustules in the superficial layers of the epidermis, acantholytic keratinocytes and neutrophilic exocytosis. We have validated the use of the anti-dsc-1 antibody in dogs, for immunhistochemistry in skin biopsies preserved in paraffin. We have observed that, in dogs with PF, the immunohistochemical pattern of desmocollin-1 was altered. Conclusions Staining of desmocollin-1 is altered in dogs with PF. We were able to corroborate the pathogenic mechanisms already demonstrated by immunohistochemistry. Thus, immunohistochemical staining for this protein may be useful in diagnosis. Its use, to study precise mechanisms, is limited and more biotechnologic tools are needed to complement the knowledge on this disease.

  

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