Desmoglein-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85709

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Desmoglein-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APPEDKVVPSFLPVDQGGSLVGRNGVGGMAKEATMKGSSSASIVKGQHEMSEMDGRWEEHRSLLSGRATQFTGATGAIMTTETTKTARATGASRDMAGAQAAAVALNEEFLRNYFTDKAASYTEEDENHTAKDCLLVYSQEETESLNAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Desmoglein-2 Antibody - BSA Free (NBP1-85709) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Desmoglein-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Desmoglein-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Desmoglein-2 Antibody [NBP1-85709]

Immunocytochemistry/ Immunofluorescence: Desmoglein-2 Antibody [NBP1-85709]

Immunocytochemistry/Immunofluorescence: Desmoglein-2 Antibody [NBP1-85709] - Staining of human cell line A-431 shows localization to plasma membrane, cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709] - Staining of human rectum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709] - Staining of human heart muscle shows moderate positivity in intercalated discs.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-85709] - Staining of human liver shows no positivity in hepatocytes as expected.
Desmoglein-2 Antibody - BSA Free Western Blot: Desmoglein-2 Antibody - BSA Free [NBP1-85709]

Western Blot: Desmoglein-2 Antibody - BSA Free [NBP1-85709]

Analysis in human cell line BEWO.
Desmoglein-2 Antibody - BSA Free Immunohistochemistry-Paraffin: Desmoglein-2 Antibody - BSA Free [NBP1-85709]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody - BSA Free [NBP1-85709]

Immunohistochemistry analysis in human rectum and liver tissues using HPA004896 antibody. Corresponding DSG2 RNA-seq data are presented for the same tissues.

Applications for Desmoglein-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmoglein-2

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.

Alternate Names

CDHF5, Desmoglein2, DSG2, HDGC

Entrez Gene IDs

1829 (Human)

Gene Symbol

DSG2

UniProt

Q14126 (Human)

Additional Desmoglein-2 Products

Product Documents for Desmoglein-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Desmoglein-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Desmoglein-2 Antibody - BSA Free

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Protocols

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