Desmoglein Family Antibody Pack

Detection of Desmoglein-1 in Human Skin Hyperplasia by Immunohistochemistry (IHC-P).

Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocyte membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Desmoglein-2 in A431 and A549 lysates by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoglein-2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Desmoglein-4 in Human Skin by Western Blot.

Western blot shows lysates of human skin tissue. PVDF Membrane was probed with 0.1 µg/mL of Human Desmoglein-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Desmoglein-4 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.