DET1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92595

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DET1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2). HVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHLNRECSLFTDDCRCVIVGSAAYLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFKCDKVVLSHNQGLYLYKNIL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DET1 Antibody - BSA Free

Western Blot: DET1 AntibodyBSA Free [NBP2-92595]

Western Blot: DET1 AntibodyBSA Free [NBP2-92595]

Western Blot: DET1 Antibody [NBP2-92595] - Western blot analysis of extracts of various cell lines, using DET1 antibody (NBP2-92595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Applications for DET1 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DET1

DET1, also known as DET1 homolog, consists of a 63.8 kDa and a 65.1 kDa isoform and is involved in the ubiquitination of target proteins as well as their consequent degradation. This protein is found in large quantities in the ovary and in small quantities in some lymphoid organs. There is currently no research with this protein and its effect on diseases. In antigen processing, immune system, and upiquitin mediated proteolysis pathways, the protein interacts with DDA1, DDB2, RFWD2, RBX1, and CUL4A.

Alternate Names

de-etiolated 1, de-etiolated homolog 1 (Arabidopsis), De-etiolated-1 homolog, DET1 homolog, FLJ10103, MGC126156, MGC126157

Gene Symbol

DET1

Additional DET1 Products

Product Documents for DET1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DET1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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