DGCR8 Antibody (3T5Q6)

Novus Biologicals | Catalog # NBP3-16306

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3T5Q6 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 436-764 of human DGCR8 (Q8WYQ5). DLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGCR8 Antibody (3T5Q6)

Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306]

Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306]

Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306] - Western blot analysis of extracts of various cell lines, using DGCR8 Rabbit mAb (NBP3-16306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306]

Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306]

Western Blot: DGCR8 Antibody (3T5Q6) [NBP3-16306] - Western blot analysis of extracts of Mouse brain, using DGCR8 Rabbit mAb (NBP3-16306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for DGCR8 Antibody (3T5Q6)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DGCR8

DGCR8 is a component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-mi

Alternate Names

C22orf12, DGCRK6chromosome 22 open reading frame 12, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8, Gy1, microprocessor complex subunit DGCR8, pasha

Gene Symbol

DGCR8

Additional DGCR8 Products

Product Documents for DGCR8 Antibody (3T5Q6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DGCR8 Antibody (3T5Q6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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